Mechanism of CRISPR-Cas system:.

Mechanism of CRISPR-Cas system:.
1. Adaptation or Immunization or Spacer acquisition is the first step, the bacteria get out DNA contamination from other organisms (foreign DNA) into cells of the image is the virus DNA and plasmid DNA, which has a red called PAM (Protospacer associated. motif) to the spacer vw of DNA that represented by the square of each color by PAM are introduced into the spacer vw DNA of foreign DNA is inserted between each repeat within CRISPR array.
2. CRISPR expression: the expression of CRISPR transcription of the CRISPR array when a long line of foreign DNA to RNA, all in the array (Pre-crRNA) then the enzyme. endoribonucleases specific cut them into small CRISPR RNA (crRNA), based on previous work and found crRNA act as silencing RNA called also called prokaryotic silencing RNA (psiRNA).
3. Interference Immunity or final step is to run to catch the Cas protein crRNA by crRNA to cut foreign DNA with base pairing with crRNA But if the foreign vw DNA mismatch between the spacer, it will not cut foreign DNA.
Summary: CRISPR-Cas vw system found in bacteria. To prevent the invasion of foreign DNA into. Especially because if the virus DNA viral DNA can be inserted into the bacterial chromosome was then. Virus will increase the number of intracellular bacteria. And the bacteria eventually die CRISPR-Cas system acts as protection from foreign DNA that bacteria vw is known to act like an immune vw system vw to bacteria. And such systems requires specificity between crRNA and foreign DNA so CRISPR-Cas system is known as adaptive immunity vw system of the bacterial CRISPR array can be transmitted from mother cell to daughter cells have. Which does not occur in the immune system of eukaryotes CRISPR-Cas system, which acts as a function of psiRNA eukaryotic RNA interference (RNAi).
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